Research Perspective Volume 6, Issue 12 pp 1010—1018

Figure 3. Effect of high density (HD) on p21- and p16-induced senescence.

HT-p21 and HT-p16 cells (see [37]) were plated at 20,000 (low density) and 600,000 (high density) cells in 6 well plates and then treated with IPTG to induce p21 and p16. After 3 days, cells were trypsinized, counted and 1000 cells were re-plated in IPTG-free medium in 6 well plates. Colonies were stained and counted after 8 days.