Research Paper Volume 7, Issue 1 pp 14—25

Functional properties of bone marrow derived multipotent mesenchymal stromal cells are altered in heart failure patients, and could be corrected by adjustment of expansion strategies

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Figure 6. Culturing strategy affects the phenotype of cellular population and the efficiency of in vitro expansion.

(A) Kaplan-Meier survival curves for analysis of proliferation rate during BM-MMSC expansion in vitro under different culture conditions. The log-rank tests confirmed the significant difference for each pair of culturing conditions (p<0,001). (∉) n=43; (■) n=20; (▲) n=21. (B-D) The frequency of SMAα+ cells in cultures seeded at low density in both, normoxia and hypoxia is reduced compared to cultures seeded high density.