Research Paper Volume 7, Issue 3 pp 152—166

Figure 1. CR serum delays senescence and extends the lifespan of normal human diploid fibroblasts.

(A) Cumulative growth curves of various normal human fibroblast lines cultured in medium containing 10% of either AL (red lines) or CR (blue lines) rat serum. Data obtained with IMR-90 (I90-79) (left panel), IMR-90 (I90-26) (middle panel) and WI-38 (right panel) are shown. PDL, population doublings (B) Representative photomicrographs of early passage IMR-90 cells (PDL 25) grown in medium with 10% FBS (upper panel) and after undergoing serial subpassages in the present of AL (left panels) or CR (right panels) rat serums are shown. Original magnification: X100.