Research Paper Volume 7, Issue 1 pp 70—81
Role of p38 mitogen-activated protein kinase in vascular endothelial aging: Interaction with Arginase-II and S6K1 signaling pathway
Figure 6. Inhibition of p38 recouples eNOS and reduces cytokine/chemokine expression in aortas/hearts of aged WT mice.
Treatment of the intact aortas and hearts of old WT mice with SB203580 (10μmol/L, 6 hours) recoupled eNOS function and reduced IL-6 and KC expression. (A) En face DHE (for detection of superoxide anion) and DAF-2DA (for detection of NO) followed by counter staining with DAPI of the aortas. n=4. (B) qRT-PCR analysis of KC and IL-6 in aortas and hearts as indicated. n=6. *p<0.05, **p<0.01, ***p<0.001 vs. control group (Con). Scale = 200 μm.