Research Paper Volume 7, Issue 2 pp 133—143

Figure 3. Maf1 mediates lifespan regulation and stress resistance of sch9Δ mutant.

(A) The extended chronological lifespan of sch9Δ mutant is shortened by MAF1 deletion. WT, sch9Δ and sch9Δ maf1Δ cells were cultured to stationary phase and the fractions of survival over indicated time points were measured by colony forming assay. Error bars stands for the SEM of 3 replicates. *** p < 0.0001. (B) BCY1 is required for sch9Δ to extend chronological lifespan. WT, sch9Δ and sch9Δ bcy1Δ cells were cultured to stationary phase and the fractions of survival over indicated time points were measured by colony forming assay. Error bars stands for the SEM of 3 replicates. *** p < 0.0001. (C) MAF1 and BCY1 are required for stress resistance of sch9Δ mutant. WT, sch9Δ, sch9Δbcy1Δ and sch9Δmaf1Δ cells were cultured to stationary phase and treated without or with H₂O₂ for 30 min. Cells were serial diluted and spotted on YPD plates and incubated for 2–3 days at 30°C. (D) Quantification of H₂O₂ resistance from 3 independent experiments as shown in C. Error bars stands for the SEM. ns, not significant; * p < 0.01, ** p < 0.01.