Research Paper Volume 7, Issue 2 pp 133—143
Distinct regulation of Maf1 for lifespan extension by Protein kinase A and Sch9
Figure 5. Maf1 is not involved in growth control by PKA and Sch9.
(A) The slow growth phenotype of sch9Δ cells is reversed by PKA hyperactivation (bcy1Δ) but not MAF1 deletion (maf1Δ). Yeast cells were streaked on YPD plates and incubated at 30 °C for 2 days. (B) The fast growing phenotype of bcy1Δ cells is not suppressed by overexpressing MAF1 (MAF1 OE). Yeast cells were streaked on SD plate with Histidine dropout and incubated at 30 °C for 3 days. (C) MAF1 overexpression does not affect tRNA synthesis in WT, sch9Δ and sch9Δbcy1Δ cells. Yeast cell growing to early log phase were metabolic labeled with 3H. Total RNA was extracted and separated by poly acrylamide gel and stained with Ethidium bromide (Etbr) as loading control. Gel was dried and autoradiographed for newly synthesized tRNA (H-3). (D) Quantification tRNA synthesis of two separate experiments shown in C. ns, not significant, ** p < 0.001.