Research Paper Volume 7, Issue 5 pp 307—318

Figure 2. Effects of the intestinal stem cell (ISC)/enteroblast (EB)-specific ATM/ATR knockdown on pS/TQ and γH2AvD signals after γ-irradiation.

(A) The pS/TQ signals after γ-irradiation in the midgut with an ISC/EB-specific knockdown of ATM, ATR, or both. Flies carrying esg^ts>GFP (a–b''), esg^ts>GFP+ATMi+ATRi (c–d''), esg^ts>GFP+ATMi (e–f''), or esg^ts>GFP+ATRi genotype (g–h'') were kept at 29 °C for 2 days and then exposed to γ-radiation. One hour after the irradiation, the gut of unirradiated (a, c, e, and g) and irradiated (b, d, f, and h) flies was excised and labeled with anti-GFP (green) and anti-pS/TQ (red) antibodies and 4′,6-diamidino-2-phenylindole (DAPI, blue). a''–h'' are merged images. (B) The γH2AvD signals after the irradiation in the midgut with an ISC/EB-specific knockdown of ATM, ATR, or both. Flies carrying the esg^ts>GFP (a–b''), esg^ts>GFP+ATMi+ATRi (c–d''), esg^ts>GFP+ATMi (e–f''), or esg^ts>GFP+ATRi genotype (g–h'') were kept at 29 °C for 2 days and exposed to γ-radiation. One hour after the irradiation, the gut of unirradiated (a, c, e, and g) and irradiated (b, d, f, and h) flies was excised and labeled with anti-GFP (green) and anti-γH2AvD (red) antibodies and DAPI (blue). a''–h'' are merged images. The original magnification is 400×.