Research Paper Volume 7, Issue 6 pp 435—449

Figure 3. The pool of DCX‐positive progenitors and the total number of developing neurons were reduced in Bmal1‐/‐ mice. Double labeling of BrdU and GFAP or DCX in DG was analyzed one day after the final BrdU administration. (A) Representative photomicrographs showing cells co‐labeled for BrdU (green) and GFAP (red). (B) Quantification of the percentage of GFAP⁺ cells among all BrdU⁺ cells. In both genotypes, the percentage of cells co‐labelled for GFAP and BrdU was equally low. (C) Representative photomicrographs showing cells co‐labeled for BrdU (green) and DCX (red). (D) Quantification of the percentage of DCX⁺ cells among all BrdU⁺ cells. The percentage of total BrdU⁺ cells expressing DCX was significantly reduced in Bmal1^‐/‐. (E) The total number of DCX⁺ cells per mm² in DG was significantly reduced in Bmal1^‐/‐. Values are represented as mean + SEM, *: P < 0.05, scale bar = 50μm.