Research Paper Volume 7, Issue 6 pp 435—449

Figure 5. Fate decision of NPCs was altered in Bmal1‐/‐ mice. Double labeling of BrdU with the marker for adult neurons NeuN and with the marker for astrocytes GFAP was analyzed 28 days after the final BrdU administration. (A) Representative photomicrographs of double labeling for BrdU (green) and NeuN (red). (B) Quantification of the percentage of NeuN⁺ cells among all BrdU⁺ cells. The percentage of BrdU/NeuN double labeled cells was significantly smaller in Bmal1^‐/‐. (C) Representative photomicrographs of double labeling for BrdU (green) and GFAP (red). (D) Quantification of the percentage of GFAP⁺ cells among all BrdU⁺ cells. The percentage of BrdU/ GFAP double labeled cells was significantly higher in Bmal1^‐/‐. Values are given as mean + SEM,*: P <0.05, ^**: P <0.01, scale bar = 50μm.