Research Paper Volume 7, Issue 7 pp 486—499
Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation
Figure 6. Western blot of lamin A/C and the expression of Zmpste24 in WT and GMF-TG mice.
(A-B) These figures show the results from western blot analyses of the lamin A/C protein in the kidney of the WT and GMF-TG mice at 10 and 60 weeks. Prelamin A was absent in the WT mice at 10 and 60 weeks (A-B; Left) and the GMF-TG mice at 10 weeks (A; Right), but it was detectable in the GMF-TG mice at 60 weeks (B; Right). There were no significant differences between the lamin C protein levels in the WT and GMF-TG mice, confirming equal loading. (C) The expression of Zmpste24 mRNA tended to decrease in the kidneys of the GMF-TG mice at 10 weeks, compared with the WT mice. The data is shown as means ± S.E. (10w WT; n=3, 10w GMF-TG; n=3). P < 0.09 vs. 10w WT mice. (D) The expression of Zmpste24 mRNA decreased in the kidneys of the GMF-TG mice at 60 weeks, compared with the WT mice. The data is shown as means ± S.E. (60w WT; n=4, 60w GMF-TG; n=4). *; P < 0.01 vs. 60w WT mice.