Attenuation of p38α MAPK stress response signaling delays the <i>in vivo</i> aging of skeletal muscle myofibers and progenitor cells

John Papaconstantinou; Chen Z. Wang; Min Zhang; San Yang; James Deford; Dmitry V. Bulavin; Naseem H. Ansari

doi:doi:http://dx.doi.org/10.18632/aging.100802

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Research Paper Volume 7, Issue 9 pp 718—733

Attenuation of p38α MAPK stress response signaling delays the in vivo aging of skeletal muscle myofibers and progenitor cells

Figure 7. (A-C) Expression levels of ALDH2, a juvenile protective factor, by the gastrocnemius of young (2 mos), middle aged (13.5 mos) and aged (20 mos) WT and DN-p38α^AF/+ mice.

(A: upper panel) The levels of expression of ALDH2 in the gastrocnemius of WT mice. (A1) young (2.0 mos); (A2) middle aged (13.5 mos); (A3) aged (20 mos) mice. (A: lower panel) The levels of expression of ALDH2 by the gastrocnemius of DN-p38α^AF/+ mice; (A4) young (2.0 mos); (A5) middle aged (13.5 mos), and (A6) aged (20 mos) mice. (B) Bar graph presentation of the western blot analyses of the data in (C) presented as the relative OD vs. β-actin levels. *p < 0.05 vs corresponding WT. (C) Western immunoblot analyses of the levels of ALDH2 in young (2.0 mos), middle aged (13.5 mos) and aged (20 mos) WT and DN-p38α^AF/+ mice. (GM, right leg). *p = 0.05 vs. WT of the same age. (D-F) Expression levels of protein-HNE complexes, COX2, and iNOS by young (3 mos) WT and DN-p38α^AF/+ mice. (D) The level of expression of protein-HNE in the gastrocnemius of young (3mos) WT mice DN-p38α^AF/+ mice (D4-6). (E) The level of expression of COX2 in the gastrocnemius of young (3mos) WT mice (E1-3) and DN-p38α^AF/+ mice (E4-6). (F) The level of expression of iNOS in the gastrocnemius of young (3mos) WT (F1-3) and DN-p38α^AF/+ (F4-6) mice.