Deletion of caspase-8 in mouse myeloid cells blocks microglia pro-inflammatory activation and confers protection in MPTP neurodegeneration model

Edel Kavanagh; Miguel Angel Burguillos; Alejandro Carrillo-Jimenez; María José Oliva-Martin; Martiniano Santiago; Johanna Rodhe; Bertrand Joseph; Jose Luis Venero

doi:doi:http://dx.doi.org/10.18632/aging.100805

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Research Paper Volume 7, Issue 9 pp 673—689

Deletion of caspase-8 in mouse myeloid cells blocks microglia pro-inflammatory activation and confers protection in MPTP neurodegeneration model

Figure 6. Caspase-8 is activated in viable CD16/32-immunolabeled microglia in substantia nigra in response to intranigral LPS injection, which is associated to higher key proinflammatory microglial markers.

Panel (a) shows illustration of dual immunofluorescence of cleaved caspase-8 and CD16/32 in substantia nigra in response to intranigral LPS in Casp8^fl/fl mice and Cre^LysMCasp8^fl/fl mice. Note how cleaved caspase-8 labeling is mostly but not completely associated to CD16/32-labeled microglia, which is decreased in Cre^LysMCasp8^fl/fl mice. In both, Casp8^fl/fl mice and Cre^LysMCasp8^fl/fl mice, some non-CD16/32-labeled cells express cleaved caspase-8. Arrows indicate viable proinflammatory CD16/32-labeled microglia expressing active caspase-8 in Casp8^fl/fl mice after MPTP. Scale bar: 30 μm.