Research Paper Volume 7, Issue 10 pp 776—787
Proteasome function is not impaired in healthy aging of the lung
Figure 1. Immunoproteasome expression is increased in lung tissue of aged mice.
(A) Overview over the proteasome active site nomenclature. (B) Representative H&E staining of lung from young and aged wildtype C57Bl/6 mice. Images from two individual animals are depicted per group. (C) RTqPCR analysis of proteasome subunit mRNA expression in young and aged wildtype mice. n = 8+SEM. (D) Western blot analysis of proteasome subunit expression in young and aged wildtype mice and quantification of protein expression relative to the β − actin loading control. Bar graphs show mean+SEM.