Research Paper Volume 7, Issue 11 pp 937—955

A comprehensive multiomics approach toward understanding the relationship between aging and dementia

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Figure 4. Increased inflammation and gliosis in the hippocampus of old SAMP8 mice are prevented by J147.

Western blot analysis of the marker for vascular endothelial inflammation VCAM-1 (A) and of the IgG (Heavy + Light chains) content (B). (C) Astrocytosis, measured by Western blot of GFAP levels. One-way ANOVA followed by Tukey-Kramer post-hoc test (n = 6/group). (D) Microgliosis was assessed by immunohistochemical (IHC) staining and number of Iba-1-positive cells per mm2 of total hippocampus calculated. Original magnification: x100. One-way ANOVA followed by Tukey-Kramer post-hoc test (n = 8/group). (E and F) Activation of the stress/inflammation-associated SAPK/JNK was measured by Western blot analysis of its phosphorylation at Thr183/Tyr185. One-way ANOVA followed by Tukey-Kramer post-hoc test (n = 6/group). All data are mean ± SD. (G) Quantitative RNA analysis of altered genes related to inflammation. Heatmap and hierarchical clustering of scaled gene expression with respective fold changes and P values for the comparisons Old/Young and Old+J147/Old. Scaled expression value (Z-score) is plotted in red-blue color scale with red indicating high expression and blue indicating low expression. One-way ANOVA followed by Tukey-Kramer post-hoc test (n = 3-4/group).