Research Paper Volume 7, Issue 11 pp 986—999
Screening of a kinase library reveals novel pro-senescence kinases and their common NF-κB-dependent transcriptional program
Figure 7. p53 or p16/Rb pathway inhibition did not revert kinase-induced senescence.
(A) One hundred thousand MRC-5 normal human fibroblasts were seeded per well in 6-well plates. The next day, the cells were infected with an E6- or E7-encoding retroviral vector and five days later they were fixed and crystal violet stained. (B) Fifty thousand MRC-5 normal human fibroblasts were seeded per well in 6-well plates. The next day, they were infected with the indicated retroviral vector. Thirteen days after infection, the cells were fixed and crystal violet stained. (C-F) MRC-5 cells were infected with the indicated retroviral vector and 5 days later, RNAs were prepared. RT-qPCR was performed to quantify (C) IL1A, (D) IL1B, (E) IL6 and (F) IL8 transcripts. Results were normalized with respect to the level of ACTB mRNA. Statistical analysis was performed with the T-test, p<0.005 ***.