Research Paper Volume 8, Issue 2 pp 366—381
Permanent farnesylation of lamin A mutants linked to progeria impairs its phosphorylation at serine 22 during interphase
Figure 3. The R50P mutation increases serine 22 phosphorylation of non-farnesylated progerin. (A) Immunofluorescence with anti-lamin A/C and anti-serine 22 phosphorylated lamin A in U-2 OS cells expressing the indicated proteins. (B) Phosphoserine 22 lamin A immunoblots in U-2 OS cells expressing the indicated proteins. (C) Immunofluorescence with anti-lamin A antibody in U-2 OS cells expressing Progerin R50P CS and treated with 1% hexanediol or vehicle. (D) Growth analysis of U-2 OS cells expressing the indicated proteins. (E) Anti-MCM6 and anti-progerin immunoblots in U-2 OS cells expressing the indicated proteins.