Research Paper Volume 8, Issue 3 pp 484—505

Figure 5. Increased SIRT4 expression is inversely associated with decreased miR-15b levels in human photoaged skin. (A) SIRT4 expression analysis in human skin samples obtained from neck vs. buttock skin of the same individuals (age ranges: 18-25 years and 60-66 years; n=15-16 per group). (B) miR-15b expression analysis in human skin samples obtained from neck vs. buttock skin of the same individuals (age ranges: 18-25 years and 60-66 years; n=9-10 per group). (C, D) Determination of SIRT4 mRNA (c) and miR-15b (d) copy numbers in the epidermal (SIRT4: n=20; miR-15b: n=20) and dermal (SIRT4: n=12-14; miR-15b: n=17-19) compartments isolated from human photoaged skin (neck vs. buttock) of the same individuals (age range: 60-66 years). Median ± SEM; *p<0.05. To evaluate statistical significance, Wilcoxon signed rank tests were employed.