Research Paper Volume 8, Issue 3 pp 521—533

Figure 3. 2-DE images and the validation of differentially abundant protein by western blot. The differentially expressed proteins in this study were defined by the gray values of the protein spots. Nine proteins were down-regulated (shown on the Tg/Vehicle gel) and 5 were up-regulated (shown on the Tg/BS-I-15 mg/kg gel) (A). To validate the proteomic results, we used a western blot to confirm 4 of the total 14 differentially expressed proteins (B). Tg/Vehicle group (M) compared with the wild-type control group (WT), * p < 0.05, ** p < 0.01; Low dose BS-I (15 mg/kg) treated group (L) compared with Tg/Vehicle group, # p < 0.05, ## p < 0.01 (C). Error bars denote mean standard error of the mean (SEM), n = 3.