Research Paper Volume 8, Issue 4 pp 810—830

Figure 5. Fkh1 instability is determined by the APC^Cdc20 complex. (A) WT and cdh1Δ FKH1-TAP cells were arrested in G1 using α-factor for 3 hours, followed by the addition of CHX. Proteins were removed every hour to determine Fkh1-TAP stability. Clb2, a known target of the APC^Cdc20 complex was also monitored as a control. (B) The experiment in (A) was repeated 3 times, with bands quantified, normalized to asynchronous controls, and plotted. Standard error and p-values are shown. (C) Asynchronous and G1 arrested WT and cdh1Δ cells were used for flow cytometry. (D) Asynchronous WT and cdc20-1 FKH1-TAP cells were treated with CHX for 1 hour. Proteins were harvested and Fkh1-TAP and Clb2 levels were determined. (E) Protein bands from three repeats of the experiment shown in (D) were quantified, normalized to - CHX samples and plotted. Standard error is shown.