Research Paper Volume 8, Issue 6 pp 1201—1215
Fat-specific Dicer deficiency accelerates aging and mitigates several effects of dietary restriction in mice
Figure 1. Metabolic changes in fat-specific Dicer knockout mice (AdicerKO). Twelve-week old mice were subjected to ad libitum (AL) or dietary restriction (DR) regimens for three months. Mice were euthanized at the end of the protocol after overnight fasting and serum (A) branched-chain amino acid (BCAA) or (B) essential amino acid (EAA) levels were assessed (N=3 per condition). Values of individual amino acids were summed, normalized by the average of the Lox AL group, Log2 transformed and Pareto scaled. Data are mean ± SE. * P < 0.05. (C-E) Gene expression in inguinal white adipose tissue (N=5-7 per condition). Mean ± SEM. ** P < 0.01, *** P < 0.001 for genotype effect; †† P < 0.01, ††† P < 0.001 for diet effect; ‡‡ P < 0.01, ‡‡‡ P < 0.001 for diet-genotype interaction.