Research Paper Volume 8, Issue 6 pp 1201—1215

Figure 2. Respiration in white adipose tissue of AdicerKO mice. Twelve-week old mice were subjected to ad libitum (AL) or dietary restriction (DR) regimens for one month. Inguinal white adipose tissue (WAT) was isolated and cytochrome c reduction was measured when NADH (A) or succinate (B) was used as substrate to assess ETC complex I-III or II-III activity, respectively (N=3-5 per condition). (C) Adipocytes were isolated and succinate-induced oxygen consumption was measured (N=4 pools of at least 2 animals per condition). (D) NADH, (E) NAD⁺ and (F) NAD⁺/NADH ratio were determined in whole WAT (representative of two independent experiments with N=2-6 per condition in each experiment). Values are mean ± SEM. * P < 0.05, ** P < 0.01 for genotype effect; †† P < 0.01 for diet effect.