Research Paper Volume 8, Issue 6 pp 1223—1235

Figure 4. Expression of CD141 and GARP on DCs renders them tolerogenic enhancing their capacity to induce T regulatory cells. DCs from young subjects were stimulated with RA at 1 μM for 24h. RA- treated DCs were sorted into CD141^hi, GARP⁺ (CD141^hi) and CD141^lo, GARP⁻ (CD141^lo) populations and cultured with allogenic CD4 T cells. (A) Flow chart for experimental set up. Bar graphs depict- (B) the level of IFN-γsecretion; (C) IL-10 secretion; (D) the percentage of CD4⁺CD25⁺CD127^lo FoxP3⁺ T reg cells; (E) Histogram depicts the expression of FoxP3 on CD4⁺CD25⁺ CD127^lo gated cells. Figures are mean ⁺/− S.E. of 5 different experiments. Purified CD141^hi and CD141^lo populations of DCs were stimulated with PAM for 24h. The expression of expression markers and cytokine secretion was determined. Bar graphs depict the Mean Fluorescence Intensity (MFI) of- (F) CD80; (G) CD86; (H) HLADR. Bar graphs depict the levels of (I) TNF-α; (J) IL-10. Figures are mean ⁺/− S.E. of 4 different experiments.