Retinoic acid treated human dendritic cells induce T regulatory cells via the expression of CD141 and GARP which is impaired with age

Sudhanshu Agrawal; Sreerupa Ganguly; Alexander Tran; Padmaja Sundaram; Anshu Agrawal

doi:doi:10.18632/aging.100973

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Research Paper Volume 8, Issue 6 pp 1223—1235

Retinoic acid treated human dendritic cells induce T regulatory cells via the expression of CD141 and GARP which is impaired with age

Figure 5. CD141^hi DCs from aged subjects are impaired in their capacity to induce T regs. DCs from aged subjects were stimulated with RA and sorted into CD141^hi, GARP⁺ (CD141^hi) and CD141^lo, GARP⁻ (CD141^lo) populations. The 2 sorted DC subsets were then cultured with allogenic CD4 T cells. Bar graphs depict- (A) the percentage of CD4⁺CD25⁺CD127^lo FoxP3⁺ T reg cells; (B) the level of IL-10 secretion; (C) IFN-γ secretion. Figures are mean ⁺/− S.E. of 6 different experiments.