Research Paper Volume 8, Issue 6 pp 1223—1235
Retinoic acid treated human dendritic cells induce T regulatory cells via the expression of CD141 and GARP which is impaired with age
Figure 6. Circulating myeloid DCs (mDCs) display similar response to RA as monocyte derived DCs. Purified mDCs from aged and young were exposed to RA for 24h. (A) Zebra plots depict the subsets of CD141+ and CD1c+ mDC populations in the young PBMCs as determined by flow cytometry. Dot plots depict the upregulation of CD141 and GARP on- (B) CD1c+ mDC subset; (C) CD141+ mDC subset in the young subjects. Figures are representative of 6 different experiments. (D) Dot blot depicts the percentage of CD141+ and CD1c+ mDC subsets in the circulation of aged and young subjects. n=12. (E) Bar graph depicts the percentage of CD141+, GARP+ CD1c mDC from aged and young subjects after treatment with RA. Figures are mean +/− S.E. of 6 experiments with different aged and young subjects.