Research Paper Volume 8, Issue 6 pp 1236—1248
Overexpressed HDAC4 is associated with poor survival and promotes tumor progression in esophageal carcinoma
Figure 1. The expression of HDAC4 is upregulated in ESCC tissues and cell lines and associated with survival. (A) Real-time qRT-PCR analysis shows that expression of HDAC4 mRNA is significantly increased in 86 ESCC tissues compared with the corresponding normal tissues. **** P <0.001; (B) The expression of HDAC4 mRNA is significantly increased in EC cell lines, EC/CUHK1, KYSE30, KYSE140, KYSE150 and KYSE180 cell lines, compared with the human normal esophageal cell line NE1. The expression was normalized by GAPDH mRNA. * P <0.05; (C) Western blot analysis of HDAC4 protein expression in eight ESCC tissues and paired normal tissues; GAPDH was used as a loading control. (D) HDAC4 protein expression in eight ESCC tissues and paired adjacent tissues was quantitated using ImageJ software (Wayne Rashband). (E) Kaplan–Meier analysis shows that HDAC4 overexpression is significantly associated with poor overall survival in 86 ESCC cases (P <0.001, log-rank test). (F) Kaplan–Meier analysis shows that expression of HDAC4 is marginally significantly associated with poor relapse-free survival in 86 ESCC cases (P =0.056, log-rank test).