Figure 2. HDAC4 silencing inhibits cell proliferation. Real-time qRT-PCR analysis of HDAC4 mRNA expression in (A) KYSE30 and (B) EC/CUHK1 cell lines transfected with HDAC4 specific siRNAs or a control siRNA. CCK8 assay was then performed to evaluate cell growth of (C) KYSE30 and (D) EC/CUHK1 cells). Each value represents the mean ± http://S.D.in three independent experiments. * P<0.05; ** P< 0.01. OD, optical density.