Research Paper Volume 8, Issue 8 pp 1608—1624

Figure 2. The SASP engenders a protumourigenic phenotype to the senescent oral fibroblasts. Soluble factors secreted by senescent fibroblasts stimulated proliferation and migration of D20 (A-B) and H357 cell lines (C-D) in 2D assay (n=3). The invasiveness of H357 cells was also increased by senescent fibroblasts in both 2D and 3D organotypic models (E-F) (n=3). Immunohistochemistry for p16^INK4A was performed in paraffin embedded sections of 3D organotypic models to confirm presence of senescent fibroblasts (F, left lower panel). All experiments were performed independently as indicated by n and with technical repeats. The bars represent mean ± SEM. *p<0.05, was determined by one-way ANOVA with post-hoc correction by Tukey test (B) and Dunn's method (D-E). Two-way repeated measure ANOVA was performed to determine statistical significance in proliferation assay (A, C) with post-hoc correction by Holm-Sidak method. (See Supplementary Figure S2).