Research Paper Volume 8, Issue 8 pp 1718—1734
Biological and biophysics aspects of metformin-induced effects: cortex mitochondrial dysfunction and promotion of toxic amyloid pre-fibrillar aggregates
Figure 3. Metformin induces accumulation of Aβ aggregates. (a) Immunofluorescence of cerebral cortex and hippocampus sections of metformin treated mice stained using anti-APP. Nuclei were stained with Hoechst 33258 and merged images with anti-APP staining are shown. (b) ThT staining of Aβ aggregates on coronary sections. High magnification of the squared areas is shown. Yellow arrows in the zoomed images indicate diffuse or punctate anti-APP staining. 20X original magnification. Scale bars = 50 μm and 5 μm in the zoomed images. n=5 per group