Research Paper Volume 8, Issue 8 pp 1703—1717

Figure 1. Induction of SA-β-Gal by IL-1β in OA chondrocytes. (A) Representative light microscopy images of cells stained with the senescent marker SA-β-Gal in monolayer cultures of OA chondrocytes. Bars: 200 μm. (B) Percentage of SA-β-Gal positive cells. OA chondrocytes were incubated with IL-1β and/or CM for 1 day and 7 days. ((C) SA-β-Gal was quantified by fluorometry. A co-culture system of AMSC and OA chondrocytes was used. Data are shown as mean±standard deviation of N=4 separate experiments with cells from separate donors. +p<0.05, ++p<0.01 with respect to non-treated cells; *p<0.05, **p<0.01 with respect to IL-1β-treated cells. FU: fluorescence units.