Research Paper Volume 8, Issue 8 pp 1703—1717

Figure 2. Effect of CM on γ-H2AX foci accumulation induced by IL-1β in OA chondrocytes. (A) Presence of γH2AX (red pixels) positive cells in a representative experiment. DAPI was used to stain the nuclei. Bars: 50μm. (B) Percentage of positive cells. (C) Presence of γH2AX (red pixels) foci in cell nucleus in a representative experiment. DAPI was used to stain the nuclei. Bars: 5μm. (D) Number of γH2AX foci per cell. OA chondrocytes were incubated with IL-1β and/or CM for 3 days. Data are shown as mean±standard deviation of N=4 separate experiments with cells from separate donors. ++p<0.01 with respect to non-treated cells; *p<0.05, **p<0.01, with respect to IL-1β-treated cells.