Research Paper Volume 8, Issue 8 pp 1703—1717
Paracrine effects of human adipose-derived mesenchymal stem cells in inflammatory stress-induced senescence features of osteoarthritic chondrocytes
Figure 7. Effect of CM on p53. (A) Immunoblotting was performed for acetylated and total p53. (B) p53 mRNA expression was determined by quantitative real-time PCR. OA chondrocytes were incubated with IL-1β and/or CM for 24 hours. Data are shown as mean±standard deviation of N=4 separate experiments with cells from separate donors. ++p<0.01 with respect to non-treated cells; *p<0.05 with respect to IL-1β-treated cells.