Research Paper Volume 8, Issue 8 pp 1703—1717
Paracrine effects of human adipose-derived mesenchymal stem cells in inflammatory stress-induced senescence features of osteoarthritic chondrocytes
Figure 8. Effect of CM on p21 and p16. (A) Protein levels of p21 were assessed by ELISA. OA chondrocytes were incubated with IL-1β and/or CM for the indicated times. (B) p21 mRNA expression was determined by quantitative real-time PCR. OA chondrocytes were incubated with IL-1β and/or CM for 24 hours. (C) Protein levels of p16 were assessed by ELISA. OA chondrocytes were incubated with IL-1β and/or CM for the indicated times. (D) p16 mRNA expression was determined by quantitative real-time PCR. OA chondrocytes were incubated with IL-1β and/or CM for 24 hours. Data are shown as mean±standard deviation of N=8 separate experiments with cells from separate donors. +p<0.05, ++p<0.01 with respect to non-treated cells; *p<0.05 with respect to IL-1β-treated cells; ##p<0.01 with respect to non-treated cells at day 1.