Research Paper Volume 8, Issue 8 pp 1703—1717
Paracrine effects of human adipose-derived mesenchymal stem cells in inflammatory stress-induced senescence features of osteoarthritic chondrocytes
Figure 9. Effect of CM on Sirt1. (A) Protein levels of Sirt1 were assessed by ELISA. OA chondrocytes were incubated with IL-1β and/or CM for the indicated times. (B) Sirt1 mRNA expression was determined by quantitative real-time PCR. OA chondrocytes were incubated with IL-1β and/or CM for 24 hours. Data are shown as mean±standard deviation of N=8 separate experiments with cells from separate donors. +p<0.05, ++p<0.01 with respect to non-treated cells; *p<0.05, **p<0.01 with respect to IL-1β-treated cells.