Research Paper Volume 8, Issue 9 pp 1876—1895

Crosstalk of clock gene expression and autophagy in aging


Figure 3. Autophagy in Cryptochrome-deficient MEFs. (A) Circadian accumulation of Bmal1 mRNA (squares) and Per2 mRNA (circles) in synchronised wild type and Cry1/2-/- MEFs. (B) Bmal1, Per2, Nr1d1 and Per1 mRNA levels in Cry1/2-/- MEFs normalised to the corresponding values in wild type MEFs. Data are shown as mean values ± SEM, n=8. (C) Autophagic flux in wild type MEFs (white) and Cry1/2-/- MEFs (black) determined by quantification of LC3-II protein levels in the absence or presence of bafilomycin (Bafilo).