Research Article Volume 8, Issue 9 pp 1952—1978

Figure 5. PPARβ/δ regulates extracellular matrix deposition and immune-cell infiltration in CNV lesions. (A) FN1 (green) and COL4 (red) immunolocalization in CNV lesions of Pparβ/δ^+/+ and Pparβ/δ^−/− mice (dotted oval demarcates the lesion area; nuclei are stained blue with Hoechst; representative images are shown; scale bar = 50 µm). (B) FN1 and COL4 staining intensity was quantified in the CNV lesions of Pparβ/δ^+/+ and Pparβ/δ^−/− mice using ImageJ (mean and S.E.M.; n = 3/group; **p < 0.01, two tailed t-test). (C) Laser CNV lesions from Pparβ/δ^−/− mice display a higher number of F4/80 (green) and Iba1 (red) immunopositive cells (dotted oval demarcates the lesion area; nuclei are stained blue with Hoechst; representative images are shown; scale bar = 50 µm). (D) The numbers of F4/80⁺ and Iba1⁺ cells in the CNV lesions of Pparβ/δ^+/+ and Pparβ/δ^−/− mice were counted using ImageJ (mean and S.E.M.; n = 3/group; *p < 0.01, two tailed t-test).