Research Article Volume 8, Issue 9 pp 1952—1978

Figure 8. Antagonism of PPARβ/δ decreases accumulation of collagen type 4 in CNV lesions. (A) FN1 (green) and COL4 (red) immunolocalization in CNV lesions of mice treated with vehicle control (1% DMSO in saline), GW0742 (0.5mg/kg/day, i.p.), or GSK0660 (1m/kg/day, i.p.) (dotted oval demarcates the lesion area; nuclei are stained blue with Hoechst; representative images are shown; scale bar = 50 µm). (B) COL4 and (C) FN1 staining intensity was quantified in the CNV lesions using ImageJ (Mean and S.E.M.; n = 3/group; *p < 0.01; ns: not significant, one way ANOVA, Tukey’s multiple comparisons test).