Research Paper Volume 8, Issue 9 pp 2153—2181

Age-related changes in cerebellar and hypothalamic function accompany non-microglial immune gene expression, altered synapse organization, and excitatory amino acid neurotransmission deficits


Figure 4. Age-associated increase in C57BL/6 cerebellar and BALB hypothalamic expression of immune transcripts. Venn diagram demonstrates that although these two regions have different cellular architectures and functions, they share considerable overlap in age-related changes in gene expression. In male C57BL/6 cerebellum, we identify 101 differentially expressed genes (DEGs), 100 upregulated in aged mice. In male BALB hypothalamus, we identify 153 DEGs, 113 upregulated in aged mice. Of note, 45 of these genes are differentially expressed in both C57BL/6 cerebellum and BALB hypothalamus; the probability of this occurring by chance is p<<0.0001. We measured gene expression with Agilent Whole Mouse Genome 4x44k arrays that use 60-mer probes to detect 41,174 full length mouse genes and ESTs. Following array quality control and normalization, we identified differentially expressed genes by log posterior odds (B) values > 0 (Supplemental Methods).