Research Paper Volume 8, Issue 10 pp 2425—2436

Figure 2. (A) Enzymatic activity of carbonic anhydrase II increases in aged brain mitochondria. The enzymatic activity of carbonic anhydrase II is higher in the 78 week old brain mitochondria compared to the 4-11 week brain mitochondria (three mitochondrial samples from individual animals in each age group and three technical replicates of each sample). Each mitochondrial sample contained 30 µg/µl of protein. (B) Carbonic anhydrase II protein levels increase in aged retina mitochondria. The protein levels of carbonic anhydrase II were measured in the murine retina mitochondria aged 4-11 weeks and 78 weeks. Carbonic anhydrase II significantly increases in retina mitochondria from older mice, p=0.028 (two-tailed unpaired t-test with Welch’s correction). Replicates were obtained from individual animals (young n=6, old n=6). Columns display mean activity ± SEM. **= p<0.03 two-tailed unpaired t-test with Welch’s correction.