Research Paper Volume 8, Issue 10 pp 2425—2436

Figure 3. (A) Carbonic anhydrase II protein levels increase in the neurodegenerative mouse model, pcd^5J cerebellum mitochondria. Carbonic anhydrase II, normalised to COXIV, is significantly increased in pcd^5J cerebellum mitochondria compared to wild type animals aged (10-13 weeks old). Replicates were obtained from individual animals (wild type n=4, pcd^5J n=4). (B) Carbonic anhydrase III protein levels are not significantly different between the pcd^5J and wild type skeletal muscle mitochondria. CAIII protein levels, normalised to GAPDH, were compared between wild type (n=3) and pcd^5J (n=3) mice aged 10-13 weeks. Replicates were obtained from individual animals. (C) Carbonic anhydrase II accumulates in the pcd^5J retina mitochondria. Carbonic anhydrase II levels are significantly higher in pcd^5J mice compared to wild type animals, p=0.0019. Replicates were obtained from individual animals (wild type n=6, pcd^5J n=6), all animals were between 9-17 weeks old. Columns display mean activity ± SEM. * = p<0.05, **= p<0.03 two-tailed unpaired t-test with Welch’s correction.