Research Paper Volume 8, Issue 11 pp 2790—2798
Expression of Rab1A is upregulated in human lung cancer and associated with tumor size and T stage
Figure 2. Rab1A is overexpressed in lung cancer cell lines but is not correlated with mTORC or MAPK expression. (A) Expression of Rab1A, P-S6K, S6K, P-AKT, AKT, P-ERK, ERK, P-MK2, MK2, P-c-Jun and c-Jun was examined in a panel of lung cancer cell lines by immunoblot. GAPDH served as a loading control. (B) Quantitative analysis of Rab1A protein levels in cell lines. The densitometry results for each Rab1A band were normalized to GAPDH, and the ratios between cancer cell lines and BEAS-2B were calculated and results represented in a histogram. Data represent means ± SD of three independent experiments. (C-F) Correlation plots of the expression level of Rab1A and P-S6K1 (T389; C), P-AKT (S473; D), P-ERK (T202/Y204; E), P-MK2 (T334; F) and P-c-Jun (S63; G). Shown are arbitrary units.