Research Paper Volume 8, Issue 11 pp 2871—2896

Figure 2. Muscle fiber-specific transgenic inhibition of NF-κB activity improves satellite cell function in an aging model. (A, B) Distribution and average of cross-sectional area of regenerating (centrally nucleated) fibers in injured TA muscle of young (n=6 mice) or aged WT (n=7 mice), or aged MISR mice (n=7 mice) at 7 days after cryoinjury. Data represented as histograms of fiber size (A) or as mean ± s.e.m. (B). P-values calculated by Kruskal-Wallis test with Step-down Bonferroni method. (C) Representative H&E staining of muscle sections taken 7 days after cryoinjury in young WT, aged WT or aged MISR mice. Scale bars, 100 μm. (D) Myogenic colony forming efficiency of satellite cells from young (n=5 mice) or aged (n=5 mice) WT or aged MISR (n=5 mice) mice. Data presented as mean ± s.e.m. P-values calculated by one-way ANOVA. (E) Frequency of satellite cells (percent of live cells by flow cytometry) in uninjured muscle of aged MISR (n=5 mice) or aged WT mice (n=5 mice). MISR mice were allowed to age alongside age-matched wild-type controls for these studies. Data presented as mean ± s.e.m. P-values calculated by Student’s t test. For all studies, young mice were 2-3 months old, and aged mice were 24 months old.