Research Paper Volume 8, Issue 11 pp 2871—2896

Figure 4. Sodium salicylate partially reverses aging-associated skeletal muscle inflammation and improves myogenic function of aged satellite cells. (A) Myogenic colony forming efficiency of satellite cells from uninjured muscle of aged WT mice receiving control (n=7 mice) or salicylate feed (n=7 mice). (B) Satellite cell frequency (percent of live cells by flow cytometry) in aged mice receiving control feed versus salicylate feed (n=11 per group). Data presented as mean ± s.e.m.; p-value calculated by Student’s t test for both (A) and (B). (C) Representative H&E staining of muscle sections taken 7 days after cryoinjury of young WT mice, or aged WT mice receiving control or salicylate feed for 6 weeks prior to injury. Salicylate treatment group continued on salicylate feed during recovery after injury. Scale bars = 100 μm. (D, E) Quantification of regenerating (centrally-nucleated) myofiber size at day 7 after cryoinjury in young WT mice or aged WT mice receiving control or salicylate feed for 6 weeks prior to injury (n=6 or 7 mice per experimental group). Salicylate treatment group continued on salicylate feed during recovery after injury. Data presented as a histogram of fiber size (D) or average fiber cross-sectional area (E). P-values were calculated by Kruskal- Wallis test and Step-down Bonferroni method for (D) and (E).