Research Paper Volume 9, Issue 1 pp 142—155

Figure 1. Characterization of phenotypic and evaluation of S1PR1/STAT3 axis in middle-aged mice. Body weight (a) (n=8 per group) and epididymal fat (b) (n=7 per group). UCP1 mRNA levels (c) and coloration of BAT (d) (n=7–10 per group). The Clams equipment was used to determinate VO₂ (e), VCO₂ (f), RER (g), and ambulatory activity point to point during 12 hours in light and dark period (h) and mean of cycle light and dark (i) (n=8 per group). Western blots showing S1PR1 protein levels and STAT3 tyrosine phosphorylation (j) in the hypothalamus of young and middle-aged mice. The mice were fasted prior to hypothalamus extraction for 10 hours (n= 6 per group). The Student’s t-test was performed to evaluate data. ± SEM are shown in (a and f) ***p<.0001; (c) **p<0,0021; (b,e and g) *p<0.05. For ambulatory activity point to point, as determined by Student´s t-test where (h) *p<0.05.