Research Paper Volume 9, Issue 1 pp 156—172

Figure 6. The effects of SM on the protein and gene expression of estrogen receptor ERα and ERβ in the uterus and vagina of mice. Western-blot (A, C) and Realtime PCR (B, D) analysis was carried out as described in the Methods. Representative blots are shown above, and quantitative analyses are shown below. P values are for one-way analysis of variance (ANOVA) comparing treatment groups with untreated mice. (A, B) ***P < 0.001, **P < 0.01 and *P < 0.05 compared with the Con group; ^###P < 0.001 and ^##P < 0.01compared with the SM group or E₂ group; ^▴▴P < 0.01 compared with the ERα. (C, D) ***P < 0.001 compared with the Sham group; ^###P < 0.001, ^##P < 0.01and ^#P < 0.05 compared with the OVX group;^∆∆∆P < 0.001 and ^∆∆P < 0.01compared with the SM group or E₂ group.