Research Paper Volume 9, Issue 5 pp 1414—1432
The protein kinase MBK-1 contributes to lifespan extension in daf-2 mutant and germline-deficient Caenorhabditis elegans
Figure 4. Loss of mbk-1 does not affect DAF-16 subcellular localization in germline-deficient C. elegans. The effect of the mbk-1 loss of function mutation mbk-1(pk1389) on subcellular localization of an intestine-specific GFP::DAF-16 protein (encoded by transgene muIs145[Pges-1::gfp::daf-16]) was determined at the times indicated in wild-type and germline-deficient glp-1(-) [glp-1(e2144ts)] animals. Images on the left were taken at 128x (48 h), 100x (60-96h) or 80x (120 h) magnification, images on the right are 6.5x magnifications of the areas boxed in red.