Research Paper Volume 10, Issue 1 pp 83—99

Resveratrol, an Nrf2 activator, ameliorates aging-related progressive renal injury


Figure 11. Small interfering RNA in HK2 cells. Representative western blots of Nrf2 and SIRT1 protein levels by transfected siRNA for Nrf2 and SIRT1 (A). Protein expression of Nrf2 and SIRT1 by transfection with siNrf2 and siSIRT1 were suppressed than the control group (B, C). Representative western blots of Nrf2, HO-1 and NQO-1 protein levels (D) and SIRT1, SOD1 and SOD2 protein levels by transfection with Nrf2 and SIRT1 (E). In groups with siNrf2 and siSIRT1 treated with resveratrol in exposure to H2O2 media, Nrf2, HO-1 and NQO-1 protein expressions were lower compared to the resveratrol group exposed H2O2 media (F-H). Similarly, the resveratrol-induced increase in protein levels of SIRT1, SOD1 and SOD2 were inhibited by siNrf2 and siSIRT1 (J-K). Quantitative analysis of the results is shown (*P<0.05, †P<0.01, ‡P<0.0001).