Research Paper Volume 10, Issue 1 pp 100—114

Effects of senescence and angiotensin II on expression and processing of amyloid precursor protein in human cerebral microvascular endothelial cells


Figure 5. The effects of BACE1 inhibitor IV on α-processing of APP in senescent human BMECs treated with Ang II. Senescent BMECs were treated with Ang II (1nM) alone or Ang II (1nM) plus BACE1 inhibitor IV (5μM) for 24h. Protein samples were subject to Western blot. Protein levels of (A) ADAM10 and (C) APP were detected, n=6-7. (B) Conditioned media (5ml) were collected for measuring sAPPα via the ELISA kit, n=8. Data are presented as mean±SEM, *P<0.05, compared to Ang II (1nM) alone.