Research Paper Volume 10, Issue 7 pp 1586—1596
Resveratrol increases resistance of mouse oocytes to postovulatory aging in vivo
Figure 4. Detection of ROS production in MII oocytes during aging in vivo. (A) Representative confocal images of DCF fluorescence in control and resveratrol-treated oocytes. ROS production was measured by fluorescent probe DCFA-DA (green). Scale bar: 100 μm. (B) The relative levels of intracellular ROS determined by quantitative fluorescence intensity. Data are expressed as mean ± SEM of at least 5 independent experiments. *Significantly different (P < 0.05).