Research Paper Volume 11, Issue 3 pp 986—1007
Shaoyao Gancao Tang (SG-Tang), a formulated Chinese medicine, reduces aggregation and exerts neuroprotection in spinocerebellar ataxia type 17 (SCA17) cell and mouse models
Figure 5. Neuroprotective effect of SG-Tang in SCA17 mice. (A) Experimental timeline. Mice received vehicle or SG-Tang (0.4% in drinking water) from 10 to 21 weeks old. Behavioral analyses were performed during (rotarod task) as well as at the beginning and the end (locomotor and footprint tasks) of the period to evaluate the treatment effect. In addition, IHC staining with 1TBP18 was performed at week 22. (B) Rotarod performance of wild type (WT) and SCA 17 transgenic (TG) mice at weeks 10, 13, 15, 17, 19, and 21 (n = 15; #: P < 0.05, ### or ***: P < 0.001). (C) Locomotor analysis of WT and SCA 17 TG mice at weeks 10 and 19 (n = 16; #: P < 0.05, ***: P < 0.001). (D) Footprint analysis of WT and SCA 17 TG mice at weeks 10, 19, and 21 (n = 14; # or *: P < 0.05, ***: P < 0.001). (E) IHC staining of TBP aggregates (red) in calbindin-positive Purkinje cells (green) of SCA17 TG mice at week 22 (n = 3).