Research Paper Volume 11, Issue 5 pp 1456—1470

Figure 7. LINC00657 enhanced cell apoptosis through indirectly regulating PTEN. (A) The predicted combined sites of PTEN and miR-190a-3p. Relative luciferase activity in LN-18 (B) and U-118MG (C) transfected with PTEN WT or PTEN MT following treatment with NC or miR-190a-3p mimics. **P<0.01, compared with NC group, unpaired t-tests. (D) Relative PTEN levels in normal tissues (adjacent tissues of GBM patients) and GBM tumor tissues (n = 40) were detected using RT-qPCR. **P<0.01, compared with adjacent tissues, paired t-tests. (E) Relative PTEN levels in in four kinds of cells including HA1800, U-87MG, LN-18 and U-118MG. *P<0.05, **P<0.01, compared with HA1800 group, unpaired t-tests. (F) Relative expressions of PTEN and active-caspase 3 in LN-18 and U-118MG transfected with pcDNA3.1-LINC00657 or pcDNA3.1-NC following treatment with NC or miR-190a-3p mimics. (G, H) Relative expressions of PTEN and active-caspase 3 in LN-18 and U-118MG cells were quantified by Image-Pro Plus. (I) Pearson's correlation scatter plot of the expressions of LINC00657 and PTEN in NSCLC tumor tissues. **P<0.01, compared with control group. ^#P<0.05, ^##P<0.01, compared with pcDNA3.1-LINC00657, unpaired t-tests.